Board Thread:Legitimate Canon Questions/@comment-4083360-20140108131256/@comment-14168656-20140108203237

Paul.rea wrote: The answer is no based on all we've seen. Peter Hale and all the members of The Alpha Pack killed people and still retained the red eyes.

If, more specifically, you're asking if the taking of an innocent life would effect Alpha eye color - we don't know since no "innocents" have been killed by an Alpha on screen to date. Peter killed his nurse but she wasn't innocent since she helped him kill Laura and the rest.

Paul, sorry if I'm missreading it, but it looks like the original question was something else (even though the OP didn't seem to realize this). The OP aked:

"Hi I was wondering if an alpha takes a life does their eyes become blue if they become beta or omegas again?"

Since the OP is asking what happens WHEN THEY BECOME A BETA OR OMEGA AGAIN, the answer is we don't have any indication at all. The only Alpha's that have been explicitly stated to revert back to Betas/Omega have been Peter, Derek, and both the Twins (even though your definition is that previews are not cannon, I'm standing by them no longer being Alpha's since Davis and the actors also alluded to this being the case).

The eyes of all three did in fact turn blue. However, for Derek we know definitively that his blue eyes were acquired BEFORE becoming an Alpha, and for Peter and the Twins it has not been revealed.

Deuc implied that he would have blue eyes were he not an alpha in Lunar Ellipse by saying that killing Jennifer 'wouldn't change the color of his eyes,' but as is the case with Peter and the Twins, we don't know if he got them before or after becoming an Alpha. And we don't have any indication that he's not still currently an Alpha.

So I think the correct answer to the question is that we don't know because the writers haven't defined it.